Whe using the Logicle scale, negative values are displayed correctly, preserving the desired symmetrical appearance of correctly compensated data.ĭata for this app note was generated using a normal whole blood sample stained with DURAClone IM Phenotyping Basic Antibody Panel* (Part Number B53309), acquired on a CytoFLEX LX flow cytometer and analyzed using Kaluza Analysis Software*. The two different scales are divided by a slider, which provides the ability to interactively control the width of each region. Changing an axis from Log to Logicle scale splits the axis into two different regions, where the positive values remain in Log scale and negative values are transformed into Linear scale. To correct this issue, Kaluza Analysis features the Logicle scale (Figure 1), which allows you to correctly display compensated data. When displaying data on a logarithmic scale (Log), correctly compensated flow cytometry data may appear to be incorrectly over-compensated because events with negative values tend to pile along the axes this distortion occurs because negative values do not exist on a Log scale.
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